In late March 2026, the MountBuzz Brazil team wrapped up fieldwork in the Atlantic Forest in Eastern Brazil, where they had spent three months revisiting sites we had worked at in 2025, and scouting for a few new sites and species. Throughout the fieldwork, the team struggled with heavy rainfalls and bad weather – which also, on the other hand, led to several exciting discoveries, such as that it is literally impossible to extract pollen through mechanical vibrations from Melastomataceae anthers when it is rainy. Likely, this inability to extract pollen comes from humidity-induced clumping of pollen grains, and makes it even more understandable why bees don’t forage under these rainy conditions! This second year’s fieldwork was structured around filling small gaps in the dataset collected last year, but primarily oriented towards new experiments, particularly single-visit pollination experiments with different bees. In single visit experiments, a previously unvisited flower is made available to a single bee visit only, and then collected in order to count pollen grains deposited on the stigma, and pollen remaining in the anthers. We will compare the remaining pollen to pollen available in unvisited flowers to obtain a measure of how much pollen the bee has removed. For these experiments, we have developed a novel test stand where we fix the flower in a standardized way and connect it to a piezo-microphone with which we can measure the tissue-borne vibrations. Amongst other things, we hope to obtain reliable measurements of vibration amplitude, which generally cannot be measured accurately under field conditions. Apart from single-visit experiments, the team was also busy performing artificial vibration experiments for as many species as possible to explore how morphologically distinct species release pollen when exposed to the full spectrum of the bee-vibration parameter space. César, who led the fieldwork, is getting ready to present preliminary findings at several conferences later this year, including the 6th Pollination Biological Symposium in Brazil in August, the 13th Colombian Botanical Congress in October, and the 40th SCAPE meeting in Sweden in October! If you are going to any of these conferences, make sure you get to listen to his talk! =)
